If the dna is in the form of a circular dna called a plasmid, it can be copied in. Base your answers to questions 31 to 33 on the information below and on your howledge of biology. Cbse worksheets for class 1 contains all the important questions on maths, english, hindi, moral science, social science, general knowledge, computers and environmental studies as per cbse syllabus. Pdf several pbluescriptderived plasmids of various sizes were constructed to study the effects of multicopy plasmid size on bacterial fitness. Partitioning is strictly controlled for low copy, but loose for high copy 8. Bacterial transformation with green fluorescent protein pglo. The purpose of this technique is to introduce a foreign plasmid into bacteria, the bacteria. Emerging patterns of plasmidhost coevolution that stabilize. Plasmid definition, types and functions biology dictionary. This will help you to prepare for what the exam will look like. Bacterial transformation with green fluorescent protein pglo genetic engineering is an umbrella term that encompasses many different techniques for moving dna between different organisms. To move the pglo plasmid dna through the cell membrane you will. Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cells transformed with the modified plasmid. Recombinant dna can be used to create massive amounts of proteins for research, and has also been used to add genes to organisms or cells.
Plasmids have played, and continue to play, indispensable roles in. Understand the uses of marker or reporter genes in molecular biology. After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the modification process. Experimental studies in molecular biology with transformed. Complete the remainder of the activity sheet you began on day 1 of the lab. In the case of dna, this is feasible for relatively short molecules such as the genomes of small viruses. A plasmid also called a vector in this context is a small circular dna molecule that replicates independently of the chromosomal dna in bacteria. Using these data, construct a restriction map of plasmid. What happens to the recombinant plasmid once inside a bacteria.
Plasmid must be unable to replicate without essential replication proteins provide in. In bacterial cells, certain autonomously replicating circular genetic elements dna are found some. Bacteria transformation activitymodeling bacteria transformation worksheet modeling bacteria transformation worksheet data and observations staple recombinant model here. Because of this effect, these substances are known as. Read a short article about how restriction enzymes are used to cut bits of dna and those bits can be inserted into the genome of other organisms. Pdf the stable maintenance of lowcopynumber plasmids in bacteria is actively driven by partition mechanisms. Plasmid mapping has revolutionized molecular biology and paved the way for the biotechnology industry. Structure of plasmid types of plasmid transfer of plasmid reproduction of plasmid 1.
To defend when attacked by a virus, bacteria use chemical weapons that break up the dna of the virus. We also acknowledge previous national science foundation support under grant numbers 1246120, 1525057, and 14739. The action of these chemicals on the viral dna is shown in the diagram below. Below is a restriction map for the plasmid pgen101 total length 20 kb. Good activity for an advanced biology or biotechnology class.
Students construct paper recombinant plasmids to simulate the methods genetic. Plasmids are small, circular, double stranded, nonchromosomal dna molecules. You will be tested on what domains plasmids can be found in and what the structure of a plasmid dna is. On an electrophoresis gel, band b is closer to the positive end of the gel than is band a. On this page you can read or download ms foglia ap biology lab cloning paper plasmid answers in pdf format. To learn about bacterial plasmids, one of the basic tools of genetic engineering to purify plasmid dna from a bacterial culture. Recombinant paper plasmids cutandpaste biotechnology. Isolating, cloning, and sequencing dna molecular biology of the. Big genetics and information transfer 3 ap central. Construct a labeled map and explain 3 points maximum h h e e 40 20 30 10 h e 40 20 30 10 e h e ecori restriction point h haeiii restriction point. This is great for homework, supplementary busy worksheet for a substitute teacher, or can be used as part. If you have no enzymes that will cut your plasmid only once, then reconstruct your plasmid. We biology lab cloning paper plasmid answer pdf on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format. Introduction to addgenes resource any newcomer who joins a molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid.
When the plasmid dna digested with combinations of restriction enzymes and the resulting fragments are electrophoresed, the following data is obtained. Biology lab cloning paper plasmid answer pdf biology lab cloning paper plasmid answer pdf are you looking for ebook biology lab cloning paper plasmid answer pdf. Once you have selected those enzymes that cut the plasmid once, start checking these enzymes against the cell dna strand. Since no plasmid was given to these bacteria, no growth is expected. Plasmid analysis is a useful method for epidemiologic typing of a variety of organisms. Compare the output tag sequences with the input tags 5. Making recombinant dna molecules is at the heart of the genetic engineering controversy. Using the information from table 4,indicate where the restriction enzyme cuts are located on the plasmid. The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. Locate the antibiotic resistant sites on the recombinant plasmid, along mth the replication site.
Although the newcomer likely knows that a plasmid is a small circular piece of dna found in bacterial cells, she may. The tools recombinant dna is a set of tools that allows scientists to move between genetics, biochemistry and. Biomedical application of plasmid dna in gene therapy. After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the. Ap biology 2007 scoring guidelines question 4 continued a using the circle provided, construct a labeled diagram of the restriction map of the plasmid. Living and nonliving things, fruits and vegetables, parts of a plant diagram, plant life cycle, life cycle of a fish, adaptation of animals, plant adaptation and life cycle of a chicken. Furthermore, within a single hostplasmid pair three distinct patterns of. Identification of bacterial plasmids based on mobility and plasmid. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and applied genetics relevant to degreelevel understanding of modern laboratory technology. Manual poses a series of questions to focus and stimulate thinking about all.
Restriction enzymes teachers guide the biology corner. Dna cloning with plasmid vectors molecular cell biology. Empirical and molecular formula worksheet an oxide of chromium is found to have the following % composition. A double digest means that you cut your plasmid with two different restriction enzymes at the same time. What is normally the source plasmid for human cloning. Ms foglia ap biology lab cloning paper plasmid answers. Gene cloning worksheet 20pts recombinant dna is a plasmid that has had foreign genes added to it. What is a plasmid and describe unique characteristics of a plasmid. I will include photos of the completed sequences when i get a chance, for now, just including answers to the analysis questions. Recover bacteria from the deceased animal spleen 4. Jun 30, 2016 on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format.
This quiz and worksheet combo can help you quickly determine your understanding of bacterial plasmids and the characteristics they have. Use a transformation solution containing cacl 2 ii. As you already have a putative map of the plasmid, you can predict which would be the expected bands. This worksheet contains basic conceptual questions about the structure of dna. If you spliced the dna gene into the middle of the plasmid replication site, the plasmid will not be able to replicate, and cannot be of use. The plasmids conveniently can be cut, fused with other dna and then.
Consult the manufacturers manual for the optimal working conditions for each enzyme. Gel electrophoresis worksheet teachers pay teachers. In outbreaks, plasmid profiling is a rapid, convenient way to follow the spread. Restriction enzymes are specific to a section of dna, depending on the base pairs at that section, you will analzye sections of dna and determine which restriction enzyme should be used. Each of the individual worksheets includes target language from the relevant lesson plan in this book. Episome in bacteria, a plasmid that can be inserted into the chromosome. The plasmid should be circular with a section of human dna spliced into the circle. The percent composition of a compound was found to be 63. Maybe dolly the sheep, or experiments carried out in molecular biology labs. Use one or more complete sentences to answer each question. Isolate plasmid dna and tag with a signature sequence 2.
By selecting the box below, i am agreeing to allow addgene, via its ebook service provider, hubspot, to store my email address indefinitely or until i opt out of receiving updates to the plasmids 101 ebook so that i can receive the plasmids 101 ebook and any updates to this ebook at the email address i. Keeping the flask with liquid agar in a water bath at 4550c can help prevent the agar from cooling too quickly. Bacteria singlecelled microbes that were one of the first types of lifeforms to evolve on earth. In a molecular biology laboratory, a student obtained competent e.
Biology chapter 12 dna and rna worksheet answer key. Inserting a plasmid into dna would allow cells to express a protein that they are lacking. Following growth of the bacteria in the presence of the antibiotic, the plasmid dna can readily be isolated from the bacterial culture. A virus or plasmid used in this way is known as a cloning vector, and the dna.
Do not let the agar cool so much that is begins to solidify. In this activity students will use a plasmid map to calculate the products of various restriction enzyme digests and predict the location of the dna fragments when run on a gel. In this biology learning exercise, students complete 7 short answer questions regarding dna, gene splicing, cloning, and bacterial proteins. Copy number is regulated by initiation of plasmid replication 10. Notice how all the fragments add up to the same total. Direct student pairs to apply their understanding of genetics to modify plasmids. Cbse worksheets all classes and all subjects printable worksheets for cbse class 1. Ap biology molecular biology lab bacterial transformation worksheet 417 below is the link to the new freeresponse booklet which shows how questions and space for answers will be distributed throughout the exam booklet.
What is the first step in the cloning of a human gene. The areas comprising the current biotechnology are bioprocess, monoclonal antibodies, and. Plasmid mapping plasmids and restriction enzymes this lesson will demonstrate the principles of plasmid mapping by examining restriction digestion patterns of plasmids used in the laboratory section of the kit and determining the position of restriction enzyme recognition sites in the plasmids by use of logic. Save the key about antibiotic resistance, from the bottom of the pink sheet for later use. Their presence influences, often dramatically, the biology of the organisms that they inhabit. Dna strands restriction enzymes 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 0 rsa i sty i both. Lab cloning paper plasmid worksheet ms foglia ap biology. Using this map as a guide, give the number of restriction fragments along with their associated lengths that. This technique allows molecular biologists to quickly evaluate the success of. Since plasmid replicon type determines inc group, the terms inc and rep type were interchangeably used to describe plasmid types. Describe a procedure to determine which bacterial cells have been successfully transformed. Plasmid replication requires host cell functions 9. Students use a paper model to simulate this reallife process used by biotechnicians. Hybridization methods are in such wide use in cell biology today that it is difficult.
A desktop resource 1st edition 2 p a g e plasmids 101. Transformation is the process by which an organism acquires and expresses a whole new gene. Our main purpose is that these 1 rna worksheet answer key photos gallery can be a guide for you, give you more samples and also bring you what you looking for. If you observe the expected sizes, then your design is correct and your analysis are confirmed. But genomes of even the simplest cells are much too large to directly analyze in detail at the molecular level. Microsoft word ap bio lab06 molecular biology worksheet wl.
Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria. This worksheet is visual and will either help drive a concept home or open their eyes to a new concept. Scientists can typically distinguish cells that have been successfully transfected with a plasmid from those that have not by a. Your recombinant plasmid should be circular with a portion of the cell dna included. Initially, inc typing relied on introduction of a plasmid into a strain carrying another plasmid and determining whether both plasmids were stably maintained in the progeny. To help teachers produce multiple colour copies for group classes, all activity worksheets are also available as a full colour pdf download from.
1546 794 643 243 616 1225 1203 78 634 1153 120 844 1443 1386 1405 552 1069 1101 980 57 761 489 521 675 1048 1011 1026 440 1336